6 protocols
AccessionType
bioassay_data_transformation
ID_REF = VALUE = GeneSpring GX ABS_CALL = DETECTION P-VALUE =
image_aquisition
GeneChips were scanned using the GeneChipĀ® Scanner 3000 7G.
feature_extraction
The data were analyzed with GeneSpring GX using Affymetrix default analysis settings and global scaling as normalization method.
hybridization
We used AffymertixGeneChip 3 IVT Express Kit for hybridization according to manufacture's protocol.
labeling
We used AffymertixGeneChip 3 IVT Express Kit for labeling according to manufacture's protocol.
nucleic_acid_extraction
Trizol extraction of total RNA was performed according to the manufacturer's instructions.