Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-45386 - Comparison of M. tuberculosis and M. bovis BCG in diluted whole blood cultures

Status
Released on 10 September 2015, last updated on 11 September 2015
Organism
Homo sapiens
Samples (3)
Array (1)
Protocols (7)
Description
Despite wide scale vaccination with Mycobacterium bovis BCG, the prevalence of tuberculosis remains high, reflecting the global variable efficacy of this vaccine against adult pulmonary TB. Characterisation of different immune responses to M. tuberculosis and M. bovis BCG would increase understanding of pathology following M. tuberculosis infection or reactivation, and would facilitate the rational design of a new vaccine. Gene expression profiling was conducted on samples from diluted whole blood cultures from three healthy donors following incubation with live mycobacteria for six days. Approximately 8,000 gene entities were at least two-fold up- or down- regulated by the mycobacteria, and both mycobacteria induced similar expression changes in approximately 2,300 genes. Strikingly, many genes exhibited qualitatively different expression patterns, with over 1,000 genes up-regulated in response to M. bovis BCG but not changed by M. tuberculosis. Gene Ontology analysis revealed that the genes which failed to upregulate in M. tuberculosis-infected cultures included a large proportion of genes with lysosomal function. The inhibited up-regulation of expression of IFN-γ-inducible protein 30, acid phosphatase 2, cathepsin B and GM2 ganglioside activator was verified in samples from six biologically independent donors by qRT-PCR. The failure to up-regulate these genes in response to M. tuberculosis may constitute an immune evasion mechanism, preventing intracellular killing and antigen presentation. Blood from three healthy BCG-vaccinated donors was diluted with growth medium and incubated alone or with live M. tuberculosis (H37Rv), M. bovis BCG for 6 days. RNA samples were pooled before hybridisation.
Experiment type
transcription profiling by array 
Contacts
Jackie Cliff <jackie.cliff@lshtm.ac.uk>, Hazel M Dockrell, Iryna N Andrade, Jacqueline M Cliff, Jang-Eun Cho, Nicholas Constantinou
Citation
Excessive Cytolytic Responses Predict Tuberculosis Relapse After Apparently Successful Treatment. Cliff JM, Cho JE, Lee JS, Ronacher K, King EC, van Helden P, Walzl G, Dockrell HM.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-45386.idf.txt
Sample and data relationshipE-GEOD-45386.sdrf.txt
Raw data (1)E-GEOD-45386.raw.1.zip
Processed data (1)E-GEOD-45386.processed.1.zip
Array designA-AFFY-44.adf.txt
Links