E-GEOD-45336 - Genome-wide methylation and expression analysis of two breast cancer cell lines [Methyl-MAPS]
Released on 15 June 2013, last updated on 4 May 2014
To improve our understanding of the relationships between methylation and expression we profiled mRNA expression and single-base resolution methylation levels for two breast cancer cell lines, MCF7 and T47D. Expression was profiled using RNA-seq. Methylation was assayed using Methyl-MAPS, which uses methylation-sensitive and -dependent restriction enzyme digests followed by high-throughput sequencing to identify methylation levels at individual CpGs (Edwards et al. 2010, Genome Research). DNA Methylation was assayed for two breast cancer cell lines using Methyl-MAPS.
methylation profiling by high throughput sequencing
Nathan VanderKraats <email@example.com>, Jeffrey F Hiken, John R Edwards, Nathan D VanderKraats
Discovering high-resolution patterns of differential DNA methylation that correlate with gene expression changes. Vanderkraats ND, Hiken JF, Decker KF, Edwards JR. , Europe PMC 23748561