E-GEOD-45296 - Reproductive status alters transcriptomic response to infection in female Drosophila melanogaster

Status
Released on 19 March 2013, last updated on 2 June 2014
Organism
Drosophila melanogaster
Samples (24)
Array (1)
Protocols (8)
Description
In this analysis, we used microarrays to contrast genome-wide transcript levels in virgin versus mated females before and after infection. We repeated the entire experiment using female mutants that do not form a germline. We found that multiple genes involved in egg production show reduced expression in response to infection, and that this reduction is stronger in virgins than it is in mated females. In germline-less females, expression of egg-production genes was predictably low and not differentially affected by infection. We also identified several immune responsive genes that are differentially induced after infection in virgins versus mated females. Eight treatment groups were analzyed, with three replicates for each treatment group.
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-45296.idf.txt
Sample and data relationshipE-GEOD-45296.sdrf.txt
Raw data (2)E-GEOD-45296.raw.1.zip, E-GEOD-45296.raw.2.zip
Processed data (1)E-GEOD-45296.processed.1.zip
Array designA-GEOD-16820.adf.txt
Links