E-GEOD-45224 - Expression data from Disulfiram or 5-FU treated EpCAM-negative non-tumor-initiating hepatocellular carcinoma (HCC) cells

Status
Released on 16 March 2013, last updated on 2 June 2014
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (7)
Description
Cancer stem cells (CSCs) or tumor-initiating cells (TICs) organize a cellular hierarchy in a similar fashion to normal stem cell systems and exhibit high tumorigenic activity in xenograft transplantation assay. Disulfiram (DSF) could preferentially eradicate TICs, but the molecular machinery of its effect against TICs still remains largely unknown. We found that DSF but not 5-FU erradicates tumor-initiating HCC cells. To compare the gene expression profiles in EpCAM-positive cells and EpCAM-negative cells, we conducted microarray analyses of EpCAM-negative HCC cells treated with DSF or 5-FU. Purified EpCAM-negative HCC cells treated with Disulfiram or 5-FU were subjected to RNA extraction and hybridization on Agilent microarrays.
Experiment type
transcription profiling by array 
Contacts
Tetsuhiro Chiba <techiba@faculty.chiba-u.jp>, Atsushi Iwama, Osamu Yokosuka, Tetsuhiro Chiba
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-45224.idf.txt
Sample and data relationshipE-GEOD-45224.sdrf.txt
Raw data (1)E-GEOD-45224.raw.1.zip
Processed data (1)E-GEOD-45224.processed.1.zip
Array designA-GEOD-13497.adf.txt
Links