E-GEOD-45184 - Gene expression signatures for bladder cancer tissues and normal adjacent tissues
Released on 15 March 2013, last updated on 2 June 2014
To identify aberrantly expressed long intergenic noncoding RNAs (lincRNAs) in bladder cancer tissues compared with normal adjacent tissues, we have employed microarray expression profiling as a discovery platform to identify lincRNAs that may play important roles in bladder cancer origin and progression. Samples of fresh frozen cancer tissues, together with normal adjacent tissues (3 cm away from the tumor), were obtained during surgical resection, and total RNA was extracted for microarray analysis. Expression of six mRNAs and lincRNAs (E2F1, CCNE2, CCNB1, lincRNA:chr1:205404014-205407007, lincRNA:chr7:130723313-130727663, lincRNA:chr4:15669186-15683175) from this signature was quantified in the same RNA samples by real-time PCR, confirming good quality of the microarray analysis. Data in the matrix are log2 transformed. Three pairs of fresh frozen bladder cancer tissues and corresponding normal adjacent tissues were used in the microarray analysis.
transcription profiling by array
Wang He <firstname.lastname@example.org>, Fenyong Sun, Guangzheng Zhong, Hao Yu, Hongyan Liu, Jian Huang, Junhua Luo, Pei Wang, Qingqing Cai, Tianxin Lin