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E-GEOD-45173 - Reprogramming by Sall4, Nanog, Esrrb and Lin28 produces high quality iPSCs with a molecular signature of developmental potency that resembles ESCs

Status
Released on 8 September 2014, last updated on 10 December 2014
Organism
Mus musculus
Samples (20)
Array (1)
Protocols (7)
Description
To reveal a gene expression signature associated with developmental competence, we selected the following groups of iPSC lines for microarray analysis. i) “Poor quality” iPSCs: This group included the three OSKM-iPSC lines Nanog-GFP OSKM#2, Oct4-GFP OSKM#2 and KH2 OSKM27, that either did not produce fully developed pups or produced very low number of pups; ii) “Good quality” iPSCs: This group included BC_2 OSKM28 and Nanog-GFP SNEL#3, both of which gave rise to live, normal pups that survived only few hours; iii) “High quality” iPSCs consisting of Nanog-GFP SNEL#2 and Oct4-GFP SNEL#1, both of which generated live pups that survived postnatally (representative pups from each iPSC group are shown in Figure S5). iv) As controls we used Nanog-GFP, Oct4-GFP and KH229 ESCs. Poor, good and high quality iPSC lines together with 3 embryonic stem cell lines were subjected to agilent microarray
Experiment type
transcription profiling by array 
Contacts
Yossi Buganim <yossib@wi.mit.edu>, Batool Akhtar-Zaidi, Prathapan Thiru, Styliani Markoulaki, Yosef Buganim, Yupeng He
Citation
The developmental potential of iPSCs is greatly influenced by reprogramming factor selection. Buganim Y, Markoulaki S, van Wietmarschen N, Hoke H, Wu T, Ganz K, Akhtar-Zaidi B, He Y, Abraham BJ, Porubsky D, Kulenkampff E, Faddah DA, Shi L, Gao Q, Sarkar S, Cohen M, Goldmann J, Nery JR, Schultz MD, Ecker JR, Xiao A, Young RA, Lansdorp PM, Jaenisch R. , PMID:25192464
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-45173.idf.txt
Sample and data relationshipE-GEOD-45173.sdrf.txt
Raw data (2)E-GEOD-45173.raw.1.zip, E-GEOD-45173.raw.2.zip
Processed data (1)E-GEOD-45173.processed.1.zip
Array designA-GEOD-13912.adf.txt
Links