ID_REF = VALUE = Robust mean Log2 intensity for replicate features calculated with S-plus/R function
Robust mean Log2 intensity for replicate features calculated with S-plus/R function. Up and down tags are processed separately and output reported as separate data sets. Note: Platform GPL8633 contains the array design which will be helpful for users who do not want to use our preprocessing strategy.
After hybridization, arrays were stained and scanned at an emission wavelength of 560 nm using a GeneChip scanner (Affymetrix)
Reactions hybridized overnight to custom-built TAG4 microarrays (Affymetrix)
The strain-specific barcodes contained in the DNA were amplified by PCR using a set of biotinylated primers
Growth in YPD (rich) media, 30 °C, 200 rpm. Measurements of optical density at 595 nm (OD595) to monitor growth
Chemical treatments incorporated into growth media, 3 concentrations with 3 biological replicates each, for 5 and 15 generations (g) of growth
Cells were collected and genomic DNA was extracted using the YDER kit (Pierce Biotechnology)