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E-GEOD-45021 - Real-time quantitative PCR analysis of rat diaphragm before and after acute cervical spinal cord injury

Status
Released on 18 December 2013, last updated on 11 June 2014
Organism
Rattus norvegicus
Samples (5)
Array (1)
Protocols (5)
Description
Male Sprague Dawley adult rats were subjected to a cervical hemisection at C2 (C2HS). Costal diaphragm (both ipsilesional and contralesional sides) were assessed under control conditions (sham surgery) and at 1 and 7 days post-C2HS. We used SA Biosciences Rat Skeletal Muscle Development and Disease RT2 Profiler PCR Array to quantitate gene expression of muscle atrophy and regeneration-relevant genes from the uninjured and injured tissues on the indicated sides and at the indicated timepoints post-lesion. qPCR gene expression profiling. Tissue was derived from the costal diaphragm from control animals, and from contralesional and ipsilesional sides at days 1 and 7 post-lesion. RNA was extracted from flash-frozen tissue (TRIzol reagent) and quantitated via spectrophotometry. Equal amount total RNA from each donor was pooled prior to gene expression analysis. N=3 or 4 rats per condition. Four genes in the array study appeared to undergo unphysiologic increases in expression following SCI: Mmp9, Leptin, activin A and alpha actinin. For these genes, expression levels in control tissue were detectable but extremely low, thus fold-increases following SCI are numerically exaggerated. Therefore, we cannot explicitly comment on the physiologic scale of this response
Experiment type
transcription profiling by RT-PCR 
Contacts
Heather H Ross <hhrunc@phhp.ufl.edu>, A R Judge, B J Dougherty, D D Fuller, H H Ross, K Z Lee, L C Gill
Citation
Rapid diaphragm atrophy following cervical spinal cord hemisection. Gill LC, Ross HH, Lee KZ, Gonzalez-Rothi EJ, Dougherty BJ, Judge AR, Fuller DD. , PMID:24341999
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-45021.idf.txt
Sample and data relationshipE-GEOD-45021.sdrf.txt
Processed data (1)E-GEOD-45021.processed.1.zip
Additional data (1)E-GEOD-45021.additional.1.zip
Array designA-GEOD-16779.adf.txt
Links