E-GEOD-44744 - Krüppel-like factor 3 KO and rescue expression data in murine embyronic fibroblasts

Status
Released on 17 October 2013, last updated on 2 June 2014
Organism
Mus musculus
Samples (4)
Array (1)
Protocols (6)
Description
Krüppel-like factor 3 (KLF3) is a transcriptional repressor that has roles in adipogenesis, B-cell maturation and erythropoiesis (for review see Pearson et al., 2012). We profiled gene expression in murine embryonic fibroblasts (MEFs) where Klf3 had been knocked-out and where the same cell had been rescued with Klf3 using microarrays. Klf3 KO murine embryonic fibroblast cell lines were produced from Klf3 KO mice. These cells were rescued with epitope tagged Klf3 (Klf3-V5) using retroviral delivery (murine stem cell virus). Stable clones were selected uner puromycin seletion and total RNA was taken for array
Experiment type
transcription profiling by array 
Contacts
Jon Burdach <geo@ncbi.nlm.nih.gov>, Alister P Funnell, Crisbel M Artuz, Lit Y Tan, Mak K Sin, Merlin Crossley, Richard C Pearson
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-44744.idf.txt
Sample and data relationshipE-GEOD-44744.sdrf.txt
Raw data (1)E-GEOD-44744.raw.1.zip
Processed data (1)E-GEOD-44744.processed.1.zip
Array designA-GEOD-11533.adf.txt
Links