E-GEOD-44715 - Mast cell expression data from murine aryl hydrocarbon receptor (AhR) null, AhR_d and normal control mice

Status
Released on 1 April 2013, last updated on 2 June 2014
Organism
Mus musculus
Samples (9)
Array (1)
Protocols (8)
Description
We found that AhR, a unique chemical sensor, is critical in controlling mast cell differentiation, growth and function in vitro and in vivo. This study seeks to further the understanding of the mechanisms of how AhR control mast cell homeostasis. Three different batches of bone marrow derived mast cells (BMMCs) from AhR null, AhR_d and normal control mice were cultured in 30% WEHI-3-conditioned medium. BMMCs were purified by negative selection with MicroBeads (Miltenyi Biotec) after 4 weeks in culture. 99% BMMCs were FcεRI+ c-kit+ CD49b- by flow cytometry analysis. The cells were further rested in cytokine free medium for 4h. Subsequently, total RNA was collected. Gene expression profiling was performed on total RNA from mast cells using Illumina Sentrix® Murine Ref8_v2_R3 BeadChips. Details of the design and the gene signatures found are given in the paper associated with this GEO Series: Yufeng Zhou, Hui-Ying Tung, Ying-Ming Tsai, Shih-Chang Hsu, Hui-Wen Chang, Hirokazu Kawasaki, Hsiao-Chun Tseng, Beverly Plunkett, Peisong Gao, Chih-Hsin Hung, Becky M. Vonakis, and Shau-Ku Huang. “Aryl hydrocarbon receptor controls murine mast cell homeostasis”, Blood 2013 Mar 5 [Epub ahead of print]
Experiment type
transcription profiling by array 
Contacts
Alan E. Berger <alanberger@alum.mit.edu>, Becky M Vonakis, Beverly Plunkett, Chih-Hsin Hung, Hirokazu Kawasaki, Hsiao-Chun Tseng, Hui-Wen Chang, Hui-Ying Tung, Peisong Gao, Shau-Ku Huang, Shih-Chang Hsu, Ying-Ming Tsai, Yufeng Zhou
Citation
Aryl hydrocarbon receptor controls murine mast cell homeostasis. Zhou Y, Tung HY, Tsai YM, Hsu SC, Chang HW, Kawasaki H, Tseng HC, Plunkett B, Gao P, Hung CH, Vonakis BM, Huang SK. , Europe PMC 23462117
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-44715.idf.txt
Sample and data relationshipE-GEOD-44715.sdrf.txt
Processed data (1)E-GEOD-44715.processed.1.zip
Additional data (1)E-GEOD-44715.additional.1.zip
Array designA-MEXP-1174.adf.txt
Links