E-GEOD-4455 - Transcription profiling of Arabidopsis roots from slim1 and the parental line grown +/-S condition

Status
Submitted on 13 March 2006, released on 13 June 2008, last updated on 27 March 2012
Organism
Arabidopsis thaliana
Samples (12)
Array (1)
Protocols (5)
Description
Plants utilize soil sulfate for production of sulfur-containing amino acids that serve as essential dietary sulfur sources for animals. Despite the global nutritional significance of this fundamental metabolic process in nature, transcription factors regulating the plant sulfur assimilation pathways have never been discovered. We isolated sulfur limitation1 (slim1) mutants from Arabidopsis, showing abnormally low expression of SULTR1;2 sulfate transporter, by screening responsiveness of SULTR1;2 promoter-GFP, as an indicator, to sulfur limitation. SLIM1 encoded an EIL-family transcription factor, EIL3. To clarify the siganificance of SLIM1 function in sulfur responsive gene expression, we analyzed the transcriptome profiles in slim1-1, slim1-2 and the parental line under +S and -S conditions. Experiment Overall Design: PSULTR1;2-GFP, slim1-1 and slim1-2 were vertically grown on the +S/-S (S1500/S15) agar medium. Root tissues of 10-day-old plants were used for RNA extraction and hybridization on Affymetrix microarrays. All conditions were duplicated.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Arabidopsis SLIM1 is a central transcriptional regulator of plant sulfur response and metabolism. Akiko Maruyama-Nakashita, Yumiko Nakamura, Takayuki Tohge, Kazuki Saito, Hideki Takahashi. Plant Cell 18(11):3235-51 (2006)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-4455.idf.txt
Sample and data relationshipE-GEOD-4455.sdrf.txt
Processed data (1)E-GEOD-4455.processed.1.zip
Array designA-AFFY-2.adf.txt
Links