9 protocols
AccessionType
array scanning protocol
Fluorescent array images were collected for both Cy3 and Cy5 with a High-Resolution C Scanner (Agilent Technologies) using Agilent Settings and image intensity data were extracted with Feature Extraction 10.5 Software using Agilent Settings
normalization data transformation protocol
Raw and background corrected data ID_REF = ID column of the reference platform gSignal = Raw Median Signal of feature in green channel rSignal = Raw Median Signal of feature in red channel gProcessedSignal = Dye-normalized signal after surrogate algorithm,green channel,used for computation of log ratio. rProcessedSignal = Dye-normalized signal after surrogate algorithm,red channel,used for computation of log ratio. VALUE = log(gProcessedSignal/rProcessedSignal) representing (test/control) INV_VALUE = log(rProcessedSignal/gProcessedSignal)
normalization data transformation protocol
Raw and background corrected data ID_REF = ID column of the reference platform gSignal = Raw Median Signal of feature in green channel rSignal = Raw Median Signal of feature in red channel gProcessedSignal = Dye-normalized signal after surrogate algorithm,green channel,used for computation of log ratio. rProcessedSignal = Dye-normalized signal after surrogate algorithm,red channel,used for computation of log ratio. VALUE = log(rProcessedSignal/gProcessedSignal)
hybridization protocol
Two-Color Microarray-Based Gene Expression Analysis Protocol
labelling protocol
Agilent's Genomic DNA ULS Labeling Kit
nucleic acid extraction protocol
Total RNA was extracted using Invitrap TwinSpin Cell Mini Kit (Stratec) and reverse transcribed using MessageAmp II Bacteria RNA Amplification Kit (Ambion)
growth protocol
Ticks were mainteined for 3 days at incubator conditons described above
sample treatment protocol
Right after molting to adult, not fed females of A. aureolatum were incubated at 35oC and 93% relative humidity (RH) for 3 days
sample treatment protocol
Right after molting to adult, not fed females of A. aureolatum were incubated at 25oC and 93% relative humidity (RH) for 3 days