E-GEOD-44274 - Gene Expression Analysis of Host Innate Immune Responses in the Central Nervous System following Lethal CVS-11 Infection in Mice
Released on 1 October 2013, last updated on 2 June 2014
The microarray and advanced pathway analysis of mice caused a paralysis in a fatal infection of a highly pathogenic CVS-11 was performed for the understanding of the detail gene networks and biological functions of inflammatory response and the related genes of innate immunity and apoptosis. Female BALB/c mice were used at 21 weeks of age. Mice were subcutaneously immunized with UV inactivated SARS-CoV (UV-V) or UV-V and Toll like receptor (TLR) ligands at 6 and 1 weeks prior to mouse-adapted SARS-CoV (n=6 per group). PBS was used in three mice as control immunization. Mice were intranasally challenged with 1E+6TCID50 in 30μL. Mice were sacrificed and collected lungs at 1 days after challenge. These mice were used for the analysis of microarray.
transcription profiling by array
Naoko Iwata-Yoshikawa <email@example.com>, A Uda, H Hasegawa, N Iwata-Yoshikawa, N Nagata, T Sata, Y Sato, Y Tsunetsugu-Yokota