Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-44238 - KDM4A regulated genes in human squamous carcinoma cells
Released on 3 May 2013, last updated on 2 June 2014
To establish a robust cellular model system for screening genes associated with cell invasion, we over-expressed the oncogenic translocated promoter region (Tpr)-MET proteins in SCC23 cells (SCC23/MET). Using a functional siRNA screen, we identified that the histone demethylase KDM4A played a critical role in the invasive growth and metastasis of SCC mediated by the Oncogenic MET. To investigate the molecular mechanism through which KDM4A inhibit the tumor cell invasion, we knock-down KDM4A in SCC23/MET cell and performed a gene microarray to examine which genes may be regulaged by kDM4A. We generated two stable cell lines. one was infected with virus containing scramble and another infected with virus infected with virus containing KDM4A shRNA. Total RNA were extracted from these two cell lines and subject to microarray.
transcription profiling by array
xiangming ding <email@example.com>, Cun-yu Wang, Xiangming Ding
Epigenetic activation of AP1 promotes squamous cell carcinoma metastasis. Ding X, Pan H, Li J, Zhong Q, Chen X, Dry SM, Wang CY. , PMID:23633675