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E-GEOD-44091 - Genome-wide expression of the epithelial layer cells of mice injected with Clostridium difficile Toxin A and B

Status
Released on 30 July 2013, last updated on 2 June 2014
Organism
Mus musculus
Samples (32)
Array (1)
Protocols (7)
Description
Toxin A (TcdA) and Toxin B (TcdB), of the pathogen Clostridium difficile, are virulence factors that cause gross pathologic changes (e.g. inflammation, secretion, and diarrhea) in the infected host, yet the molecular and cellular pathways leading to observed host responses are poorly understood. To address this gap, TcdA and/or TcdB were injected into the ceca of mice and the genome-wide transcriptional response of epithelial layer cells was examined. Bioinformatic analysis of gene expression identified sets of cooperatively expressed genes. Further analysis of inflammation associated genes revealed dynamic chemokine responses. In total, 32 samples were analyzed (one microarray per mouse). The sample groups are separated by two factors: Toxin (TcdA, TcdB, TcdA+TcdB, or Sham injection) and endpoint (2, 6, or 16h). TcdA+B at 16h was not included, leaving 11 sample groups. Each sample group included biological triplicates, except TcdA+TcdB at 6h (one array) and Sham injection at 16h (four arrays).
Experiment type
transcription profiling by array 
Contacts
Kevin Michael D'Auria <kd3jd@virginia.edu>, Cirle A Warren, Erik L Hewlett, Gina M Donato, Glynis L Kolling, Jason A Papin, Kevin M D'Auria, Mary C Gray
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-44091.idf.txt
Sample and data relationshipE-GEOD-44091.sdrf.txt
Processed data (1)E-GEOD-44091.processed.1.zip
Array designA-AFFY-45.adf.txt
Links