E-GEOD-43998 - C/EBPa gene signature
Released on 1 April 2013, last updated on 2 June 2014
We defined the C/EBPa signature characterized by a set of genes which are upregulated upon C/EBPa activation. In order to identify the C/EBPa signature, we performed microarray gene expression analysis of K562 cells stably expressing p42-C/EBPa-ER after activating the C/EBPa construct to translocate to the nucleus for 6 hours with beta-estradiol. The gene expression profile was performed in K562 p42-C/EBPa-ER expressing cells treated with beta-estradiol (n=4) or EtOH vehicle control (n-4) for 6 hours. Induction of nuclear localization of C/EBPa-ER fusion protein was achieved by addition of 1 uM beta-estradiol as indicated into the culture medium.
transcription profiling by array
Daniel G Tenen <email@example.com>, Adam L Liss, Chen Ju, Chia H Ooi, Hanna Radomska, Martin Balastik, Mengchu Wu, Meritxell Alberich-Jordà, Patrick Tan, Polina Zjablovskaja, Ruud Delwel, Tomas Brdicka, Touati Benoukraf