4 protocols
normalization data transformation protocol
Sequence tags were processed and analyzed using BioScope software package (Applied Biosystems) MicroRNA sequence tags were normalized by thousand mappable tags Genome_build: hg19 Supplementary_files_format_and_content: Tab delimited file of microRNA sequence tags per thousand mappable tags
nucleic acid library construction protocol
RNA was purified and size fractionated using miRNEASY RNA isolation kit and Rneasy MinElute Clean up kit (Qiagen) Libraries were constructed using 1 microgram of small RNA and the Small RNA Expression Kit (Applied Biosystems)
sample treatment protocol
Cells were either mock infected or infected with Sendai virus (5 pfu/cell) for 10 hours
growth protocol
A549 cells were cultured in F12K media (Gibco) supplemented with 10% cosmic calf serum (Hyclone) and pennecilin and streptomycin