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E-GEOD-43928 - Expression data from TNF-stimulated mouse glomeruli

Status
Released on 11 September 2013, last updated on 2 June 2014
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (7)
Description
The specific contribution of the two TNF-receptors Tnfr1 and Tnfr2 to TNF-induced inflammation in the glomerulus is unknown. In mice, TNF exposure induces glomerular expression of inflammatory mediators like adhesion molecules and chemokines in vivo, and glomerular accumulation of leukocytes. To examine Tnfr-specific inflammatory responses in intrinsic glomerular cells but not infiltrating leukocytes we performed microarray gene expression profiling on intact glomeruli isolated from wild-type and Tnfr-deficient mice following TNF exposure in vitro. Glomeruli were isolated from male C57BL/6J wild-type and Tnfr-deficient mice applying a magnetic bead-based isolation technique. Isolated intact glomeruli were stimulated with TNF for 12 hours in vitro for subsequent RNA extraction and hybridization on Affymetrix microarrays.
Experiment type
transcription profiling by array 
Contacts
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-43928.idf.txt
Sample and data relationshipE-GEOD-43928.sdrf.txt
Raw data (1)E-GEOD-43928.raw.1.zip
Processed data (1)E-GEOD-43928.processed.1.zip
Array designA-AFFY-45.adf.txt
Links