2 protocols
normalization data transformation protocol
ChIP-seq reads were aligned to the mm9 genome assembly using BWA v0.5.9 Peaks were called using MACS version 1.4.1 with the following setting: mfold=10,30, bandwith=300, p-value cutoff=1E-5. Genome_build: NCBI37/mm9 Supplementary_files_format_and_content: BED files
nucleic acid library construction protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with specific antibodies. Libraries were prepared according to Illumina's instructions using the TruSeq DNA sample preparation kit. The ChIP-seq DNA libraries were sequenced as single 50bp reads using an Illumina Hiseq 2000 sequencer.