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E-GEOD-43633 - Acute hepatic transcriptional response in male mice following oral exposure to benzo(a)pyrene

Status
Released on 1 July 2015, last updated on 19 August 2015
Organism
Mus musculus
Samples (92)
Array (1)
Protocols (7)
Description
Benzo(a)pyrene is a well-established human carcinogen in humans and rodents. In the present study, we sought to determine the dose- and time-dependent changes in gene expression upon oral exposure to benzo(a)pyrene. Adult male B6C3F1 mice were exposed to four doses of benzo(a)pyrene or vehicle control for three days and sacrificed 4 or 24 hours after the final exposure. This experiment examined the hepatic transcriptional response of male mice exposed to BaP for 3 days at four different doses, including D1 (300 mg/kg BW/day), D2 (150 mg/kg BW/day), D3 (50 mg/kg BW/day) and D4 (5 mg/kg BW/day), and one control. Each dose group was further examined at 2 time points, 4 hours and 24 hours, following the final exposure. Each dose group and time point had 4-5 biological replicates. There were a total 46 samples (arrays) included in the final analysis using a two-colour reference design.
Experiment type
transcription profiling by array 
Contacts
Sarah Labib <geo@ncbi.nlm.nih.gov>, Amal I Malik, Andrew Williams, Byron Kuo, Carole L Yauk, France Lemieux, Ivy D Moffat, Julie A Bourdon, Julie K Buick, Kelly Jackson, Mirjam Luijten, Nikolai L Chepelev, Sabina Halappanavar
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-43633.idf.txt
Sample and data relationshipE-GEOD-43633.sdrf.txt
Raw data (2)E-GEOD-43633.raw.1.zip, E-GEOD-43633.raw.2.zip
Processed data (1)E-GEOD-43633.processed.1.zip
Additional data (1)E-GEOD-43633.additional.1.zip
Array designA-MEXP-724.adf.txt
Links