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E-GEOD-43514 - Xylose metabolism in Candida utilis

Status
Released on 1 January 2014, last updated on 2 June 2014
Organism
Cyberlindnera jadinii
Samples (8)
Array (1)
Protocols (6)
Description
A recombinant C. utilis strain expressing Candida shehatae xylose reductase K275R/N277D (NADH-preferring), C. shehatae xylitol dehydrogenase and Pichia stipitis xylulokinase produce ethanol from xylose. Here, we report the transcriptional-profiling in the engineered C. utilis strain grown on xylose using DNA microarray. Transcriptome analysis indicated that expression of genes encoding the tricarboxylic acid cycle, respiration enzymes and the ethanol consumption were increased significantly when cells were cultivated on xylose. Gene expression in Candida utilis cells grown on glucose or xylose was measured at 10.5 and 24 hours, respectively. Two or three independent experiments were performed at each time for each experiment.
Experiment type
transcription profiling by array 
Contacts
Hideyuki Tamakawa, Yasuyuki Tomita
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-43514.idf.txt
Sample and data relationshipE-GEOD-43514.sdrf.txt
Raw data (1)E-GEOD-43514.raw.1.zip
Processed data (1)E-GEOD-43514.processed.1.zip
Array designA-GEOD-16510.adf.txt
Links