8 protocols
AccessionType
bioassay_data_transformation
ID_REF = VALUE = Normalized gene level expression from GC-RMA
image_aquisition
Affymetrix protocol
feature_extraction
GC-RMA was performed in R for normalization and background correction.
hybridization
Affymetrix protocol
labeling
Affymetrix protocol
grow
Incubated at 28C
nucleic_acid_extraction
RNA was extracted with Trizol and submitted to the Microarray Core Facility at the Molecular Genetics Department at Children's Hospital Boston/ Harvard Medical School
specified_biomaterial_action
Embroys grown in 1% glucose in E3 media from 12-36hpf