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E-GEOD-42899 - Transcription profiling by high throughput sequencing of Arabidopsis roots after magnesium treatment

Status
Released on 14 December 2012, last updated on 22 February 2016
Organism
Arabidopsis thaliana
Samples (2)
Protocols (4)
Description
Physiological mechanisms involved in root hair development in response to magnesium (Mg) availability are unclear. This study investigated the influence of Mg availability on root hair development in arabidopsis grown in different Mg concentrations ranging from 0.5 μM to 10 mM. After 7-d treatment, root hair development was enhanced in roots exposed to low Mg but was inhibited severely in roots grown in high Mg. Low Mg (0.5 µM) enhanced many genes like LRX1, COW1, EXP7 and ROP2 that control root hair development. Low Mg supply also increased concentrations of total Ca2+ and ROS in roots, but application of either BAPTA or DPI to low Mg treatment blocked the enhanced development of root hairs. The opposite was true when the plants under high Mg (3 mM) were supplied with Ca2+ or PMS. Besides, in roots under low Mg and high Mg, the most significant biological function enrichment were in the ‘oxidation reduction’, ‘cell wall organization’, ‘ion response’. This study demonstrated that Ca2+ and ROS played critically in controlling the Mg-induced development of root hairs. Meanwhile, transcriptome analysis associated with Mg supply contributed to a better understanding of molecular events responsible for sensing Mg status. Roots were sampled from five-week-cultivated Arabidopsis after 7 d treatment of low Mg (supplied with 0.5 μM Mg2+) and high Mg (supplied with 10 mM Mg2+).
Experiment type
RNA-seq of coding RNA 
Contacts
Caixian Tang, Gulei Jin, Lijuan Liu, Miao Liu, Rushan Cai, Yaofang Niu, Yongsong Zhang
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-42899.idf.txt
Sample and data relationshipE-GEOD-42899.sdrf.txt
Processed data (1)E-GEOD-42899.processed.1.zip
Links