E-GEOD-42833 - Analysis of transcriptional response to mutated Potyvirus Potato virus A that induces necrotic symptoms
Released on 23 March 2013, last updated on 31 March 2013
Mutations introduced to a highly variable region in HCpro reduced HCpro-HIP2 interactions and virulence of PVA, and induced novel types of necrotic symptoms in the systemically infected leaves of Nicotiana species. Microarray-based analysis of gene expression revealed induction of large numbers of defence-related genes including jasmonic acid and ethylene inducible pathways in the leaves systemically infected by the mutated PVA. Three treaments: infection with wild type PVA (WT), infection with mutated PVA (MUT), and mock-inoculation (Mock). Six plants were treated for each treatment. Experiment was repeated three times. WT and MUT samples from all three experiments were hybridised on microarray. Mock samples from experiments 1 and 2 were hybridised as healthy controls.
transcription profiling by array
Jari P Valkonen, Minna-Liisa Rajamaki, Tuuli Haikonen, Yan-Ping Tian
Mutation of a Short Variable Region in HCpro Protein of Potato Virus A Affects Interactions with a Microtubule-Associated Protein and Induces Necrotic Responses in Tobacco. Haikonen T, Rajamï¿½ki ML, Tian YP, Valkonen JP. , Europe PMC 23514111