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E-GEOD-42672 - Conversion of human fibroblast to endothelial cell by defined factors

Status
Released on 3 December 2012, last updated on 13 May 2014
Organism
Homo sapiens
Samples (4)
Array (1)
Protocols (8)
Description
Transient pluripotency-factor-based signaling-directed (TPS) transdifferentiation approach could be further applied to generate functional induced endothelial (iEnd) cells from human fibroblasts with only two factors: Oct4 and Klf4 (OK). The iEnd cells exhibit characteristic endothelial cell phenotype in vitro and in vivo and are capable of functionally promoting vascular regeneration and blood perfusion in a murine model of PAD. Human fibroblasts were transfected with Oct4 and Klf4 then changed to endothelial cell culture media with growth factors. iENDs were purified by FACS and enriched in EGM2 media.
Experiment type
transcription profiling by array 
Contacts
jun li <jun-li04@mails.tsinghua.edu.cn>, Jun Li, Ngan Huang, Sheng Ding
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-42672.idf.txt
Sample and data relationshipE-GEOD-42672.sdrf.txt
Raw data (1)E-GEOD-42672.raw.1.zip
Processed data (1)E-GEOD-42672.processed.1.zip
Array designA-AFFY-44.adf.txt
Links