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E-GEOD-42595 - Gene expression changes induced by the pan-PIM inhibitor ETP-39010 in 4 peripheral T cell lymphoma cell lines

Status
Released on 14 November 2014, last updated on 28 November 2014
Organism
Homo sapiens
Samples (96)
Array (1)
Protocols (7)
Description
Peripheral T cell lymphoma (PTCL) is a very aggressive disease which currently lacks efficient targeted therapy. New therapeutic strategies are needed to improve the very poor outcome of these patients. In these study we hypothesized that PIM kinases could be of therapeutic value in PTCL because we found an overexpression of PIM1 and PIM2, but not PIM3, in PTCL cases, cell lines and primary tumoral T cells from Sezary Syndrome patients, compared with reactive lymph nodes and normal T cells from healthy donors, respectively. In order to understand the mechanism of action of this pan-PIM inhibitor in PTCL, 4 cell lines (DERL7, HuT78, SR786 and MyLa) were treated with 10 μM of ETP-39010 for 0, 2, 4, 6, 10 and 24 h, and gene expression profiling was performed. 4 PTCL cell lines (DERL7, HuT78, SR786 and MyLa) were treated with DMSO and 10 μM of the pan-PIM inhibitor ETP-39010, and hybridized using the Universal Human Reference RNA (Stratagene, La Jolla, CA) as the reference sample.
Experiment type
transcription profiling by array 
Contacts
Esperanza Martín-Sánchez <geo@ncbi.nlm.nih.gov>, Miguel A Piris, Socorro M Rodríguez-Pinilla
Citation
PIM Kinases as Potential Therapeutic Targets in a Subset of Peripheral T Cell Lymphoma Cases. Mart�n-S�nchez E, Odqvist L, Rodr�guez-Pinilla SM, S�nchez-Beato M, Roncador G, Dom�nguez-Gonz�lez B, Blanco-Aparicio C, Garc�a Collazo AM, Cantalapiedra EG, Fern�ndez JP, Olmo SC, Pisonero H, Madureira R, Almaraz C, Mollejo M, Alves FJ, Men�rguez J, Gonz�lez-Palacios F, Rodr�guez-Peralto JL, Ortiz-Romero PL, Real FX, Garc�a JF, Bischoff JR, Piris MA. , PMID:25386922
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-42595.idf.txt
Sample and data relationshipE-GEOD-42595.sdrf.txt
Raw data (2)E-GEOD-42595.raw.1.zip, E-GEOD-42595.raw.2.zip
Processed data (1)E-GEOD-42595.processed.1.zip
Array designA-AGIL-28.adf.txt
Links