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E-GEOD-42498 - Deletion of Cebpa from HSCs

Status
Released on 10 December 2013, last updated on 13 January 2014
Organism
Mus musculus
Samples (7)
Array (1)
Protocols (7)
Description
In this study, we use a conditional mouse model for Cebpa to investigate the significance of C/EBPα in HSCs. The frequency of HSCs is unaltered following deletion of C/EBPα, however, upon serial transplantations of either full BM or purified HSCs, the stem cells and stem cell activity is lost. This is not due to increased proliferation, but rather caused by a shift from quiescence to apoptosis with a resultant exhaustion of the stem cell pool. We identify direct C/EBPα target genes by combining genome-wide C/EBPα ChIP-seq analysis in stem and progenitor cells with gene expression data from HSC with and without C/EBPα. Furthermore, we explore the impact of C/EBPα on active and repressive histone modifications by doing functional genome-wide ChIP-seq analysis of H3K4Me3 and H3K27Me3 in stem and progenitor cells with and without C/EBPα. We have sorted HSCs from 3 Cebpaflox/flox and 4 Cebpaflox/flox;Mx1Cre mice 18 days after pIC injection.
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-42498.idf.txt
Sample and data relationshipE-GEOD-42498.sdrf.txt
Raw data (1)E-GEOD-42498.raw.1.zip
Processed data (1)E-GEOD-42498.processed.1.zip
Array designA-AFFY-130.adf.txt
Links