E-GEOD-4246 - Transcription profiling of human HEK-293 cells to identify Dicer and Argonaute regulated gene expression

Status
Submitted on 15 February 2006, released on 12 June 2008, last updated on 27 March 2012
Organism
Homo sapiens
Samples (38)
Array (1)
Protocols (2)
Description
HEK-293-derived cell lines depleted of Dicer or one of four Argonaute proteins were generated. Microarray analysis of these knock down cell lines and various control cell lines was performed to address how large the proportion of genes is that are regulated by RNA silencing in an individual cell type. Comparison of different arrays revealed commonly (up) regulated genes. The highest overlap was observed in Ago2 and Dicer knockdown cells. Experiment Overall Design: three control cell lines (T-Rex, shRNA, empty vector), two induvidual Argonaute 1 and 4 (Ago1kd, Ago4kd) knock down cell lines, one Argonaute 2 and 3 (Ago2kd, Ago3kd) and two induvidual Dicer knock down cell lines were analyzed as biological replicates
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Effects of Dicer and Argonaute down-regulation on mRNA levels in human HEK293 cells. Daniela Schmitter, Jody Filkowski, Alain Sewer, Ramesh S Pillai, Edward J Oakeley, Mihaela Zavolan, Petr Svoboda, Witold Filipowicz. Nucleic Acids Res 34(17):4801-15 (2006)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-4246.idf.txt
Sample and data relationshipE-GEOD-4246.sdrf.txt
Processed data (1)E-GEOD-4246.processed.1.zip
Array designA-AFFY-44.adf.txt
Links