Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-42431 - Mechanisms of response to silver nanoparticles Enchytraeus albidus (Oligochaeta): survival, reproduction and gene expression profile

Status
Released on 22 May 2013, last updated on 2 June 2014
Organism
Enchytraeus albidus
Samples (15)
Array (1)
Protocols (6)
Description
Due to its antimicrobial activity, silver nanoparticles (Ag-NPs) are among the most used NPs worldwide, yet little information is available regarding their effects, particularly in soil dwelling organisms. Enchytraeids (Oligochaeta) are important members of the soil fauna which actively contribute to the acceleration of organic matter decomposition and nutrient recycling processes. Hence, for hazard and risk assessment it is important to provide toxicity data for these organisms and to understand more in regard to the mode of action of Ag-NPs within organism. To study this we conducted toxicity experiments using the OECD standard guideline, testing Ag-NPs and AgNO3, having assessed survival, reproduction and differential gene expression. Population toxicity responses were assessed showing higher toxicity for the AgNO3. In an attempt to understand the mode of action we performed transcription profiling using the microarray. Gene expression profile of Enchytraeus albidus was analysed after 2 days of exposure to 100 and 200 mg/kg of two silver forms (nanoparticles and salt_silver nitrate) in OECD soil. Three biological replicates per test treatment and control (clean OECD soil) were used.
Experiment type
transcription profiling by array 
Contacts
Susana Isabel Lopes Gomes <susana.ilopes.gomes@gmail.com>, Amadeu Soares, Janeck Scott-Fordsmand, Mónica Amorim, Susana Gomes
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-42431.idf.txt
Sample and data relationshipE-GEOD-42431.sdrf.txt
Raw data (1)E-GEOD-42431.raw.1.zip
Processed data (1)E-GEOD-42431.processed.1.zip
Array designA-GEOD-14928.adf.txt
Links