E-GEOD-42279 - AAPH-treated blastocysts vs CTL-treated blastocysts

Status
Released on 14 November 2012, last updated on 3 May 2014
Organism
Bos taurus
Samples (16)
Array (1)
Protocols (9)
Description
In order to understand how in vitro culture affects embryonic quality, we analyzed survival and global gene expression in bovine blastocysts after exposure to increased oxidative stress conditions of IVC. A pro-oxidant agents that act extra-cellularly by promoting ROS production (0.01 mM 2,2'-azobis (2-amidinopropane) dihydrochloride [AAPH]) was added from days 3 to 7, and transcriptomic analysis was then performed in resulting blastocysts. Precisely, after in vitro maturation and fertilization, bovine zygotes were culture in vitro in normal condition, then at day 3, embryos were allocated into culture in control or supplemented with AAPH (0.01 mM) until day 7. At this time, blastocysts were harvested and analyzed. Our hypothesis was that AAPH treatment will affect blastocyst survival and gene expression associated with low embryo quality 4 replicates of 10 blast/rep was produced for AAPH and control treatment. According to a dye swap design with 2 colors, 4 arrays were used to compare AAPH (green) against CTL (red) and 4 arrays were used to compared AAPH (red) againt CTL (green).
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-42279.idf.txt
Sample and data relationshipE-GEOD-42279.sdrf.txt
Raw data (1)E-GEOD-42279.raw.1.zip
Processed data (1)E-GEOD-42279.processed.1.zip
Array designA-GEOD-13226.adf.txt
Links