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E-GEOD-42251 - Effects of EVI1 and EVI1Δ324 mild expression in HeLa cells

Status
Released on 14 November 2012, last updated on 20 November 2012
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (8)
Description
We studied the variations of mRNA amounts after Flag-EVI1, Flag-EVI1Δ324, or Flag expression in HeLa cells. Despites EVI1 discovery in 1988, its recognized role as a dominant oncogene in myeloid leukemia and more recently in epithelial cancers, only a few target genes were known and it was not clear why EVI1 was involved in cancer progression. Here we obtained the genomic binding occupancy and expression data for EVI1 in human cells. We identified numerous EVI1 target cancer genes and genes controlling cell migration and adhesion. Moreover, we characterized a transcriptional cooperation between AP1 and EVI1 that regulated proliferation and adhesion through a feed-forward loop. This study provides human genome-wide mapping and expression analyses for EVI1 that will be useful for the research community. 12 samples were collected. Each condition was done in 4 replicates, collected 24 hours after transfection (for mild expression of EVI1 or EVI1Δ324). Transfections with Flag-expressing vector were used as controls.
Experiment type
transcription profiling by array 
Contacts
Ahmed Sayadi <ahmeds@imcb.a-star.edu.sg>, A Sayadi, Emilie A Bard-Chapeau, Guillaume Bourque, Justin Jeyakani, Neal G Copeland
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-42251.idf.txt
Sample and data relationshipE-GEOD-42251.sdrf.txt
Raw data (1)E-GEOD-42251.raw.1.zip
Processed data (1)E-GEOD-42251.processed.1.zip
Array designA-AFFY-141.adf.txt
Links