E-GEOD-42222 - HeLa cells consist of two cell types, as evidenced by cytochemical staining for alkaline phosphatase activity: A possible model for cancer stem cell study

Released on 23 April 2013, last updated on 2 June 2014
Homo sapiens
Samples (2)
Array (1)
Protocols (7)
Alkaline phosphatase (ALP) is known to be a marker for several somatic stem cells and cancer cells. We found that human squamous cell carcinoma HeLa cells are comprised by ALP-positive and negative cells. Single cell-derived colony assay revealed that the former cells are labile with respect to ALP activity, but the latter are stable. We cloned ALP-negative cells from the HeLa cells, and named H-1 clone. DNA microarray analysis revealed that gene expression pattern of H-1 cells is almost the same with that of their parental HeLa cells, but several genes for glycoprotein hormone alpha chain, ras-related and estrogen-regulated growth inhibitor, ALP, and Frizzled-10 was respectively 18.2, 9.6, 9.2 and 10.5–fold are upregulated in the HeLa cells. Although there is no evidence that the ALP-positive cells are cancer stem cells (CSCs) at present, HeLa cells comprised by ALP-positive and -negative cells may be a good model for CSC study in future. HeLa cells consist of two cell types, namely alkaline phosphatase (ALP)-positive and negative cells. To distinguish gene expression pattern between these two types of cells, microarray analysis was performed using HeLa cells (parental cell line; a mixture of ALP-positive and negative cells) and H-1 cells, ALP-negative cells derived from HeLa cells
Experiment type
transcription profiling by array 
Emi Inada, Issei Saitoh, Masahiro Sato, Masato Ohtsuka, Naoko Kubota, Satoshi Watanabe, Shingo Nakamura, Takayuki Sakurai
Investigation descriptionE-GEOD-42222.idf.txt
Sample and data relationshipE-GEOD-42222.sdrf.txt
Raw data (1)E-GEOD-42222.raw.1.zip
Processed data (1)E-GEOD-42222.processed.1.zip
Array designA-AFFY-44.adf.txt