normalization data transformation protocol
Samples were GC-RMA normalized in Anduril bioinformatics workflow engine using R ID_REF = VALUE = gene level, GC-RMA data
array scanning protocol
GeneChip® Scanner 3000 7G with AutoLoader was used to scan the arrays
Samples were processed with Affymetrix GeneChip 3’ IVT Express Kit according to manufacturer's instructions and hybridized to GeneChip Human Genome U133 plus 2.0 Array (all Affymetrix, Santa Clara, California, USA) at +45°C according to GeneChip® Expression Analysis Technical Manual (With Specific Protocols for Using the GeneChip® Hybridization, Wash and Stain Kit)
Labeling was performed according to protocols from Affymetrix.
HMEC-1 were cultured in EBM-2 medium with EGM-2 SingleQuots (Lonza, Basel, Switzerland). TIME were cultured according to ATCC instructions (CRL-4025). Cells were subcultured after reaching ~80% confluency.
sample treatment protocol
Cells were not treated in any way
nucleic acid extraction protocol
Total RNA was isolated using a NucleoSpin RNA isolation kit (Macherey&Nagel, Düren, Germany)