7 protocols
AccessionType
bioassay_data_transformation
ID_REF = VALUE = Genespring signal intensity
nucleic_acid_extraction
Quiagen Rneasy kit for isolated cells was used as per manufacturer's protocol
image_aquisition
GeneChips were scanned using the Affymetrix GeneChip scanner 3000.
labeling
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5ug of total RNA
feature_extraction
Data was analyzed with Genespring 9.0 software using default analysis settings. No minumium fluorescence cut-off was used for data analysis.
hybridization
Following fragmentation, 15ug of cRNA were hybridized for 16 hrs at 45C on U133 2.0 Human GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
specified_biomaterial_action
Whole PBMCs were isolated from blood and then CD11c+ cells were sorted using magnetic beads bound to human anti-CD11c+ antibody.