E-GEOD-41963 - Gene expression analysis in wild-type and OsGRX8 overexpression line in response to various treatments

Status
Released on 23 September 2013, last updated on 2 June 2014
Organism
Arabidopsis thaliana
Samples (16)
Array (1)
Protocols (7)
Description
Glutaredoxins (GRXs) are the ubiquitous oxidoreductase enzymes, which play important role in defense against various stresses. To analyze the function of a CC-type rice GRX gene, OsGRX8, we overexpressed it into Arabidopsis constitutively. The physiological analyses revealed that overexpression of GRX gene enhanced abiotic stress tolerance in transgenic plants as compared to wild-type. We used microarrays to study the global effect of overexpression of OsGRX8 in Arabidopsis under control and various treatments. Wild-type and transgenic Arabidopsis seedlings were grown on MS medium for 14-days in a culture room with a daily photoperiodic cycle of 16h light and 8h dark. Seedlings were incubated in water (control) or 100 µM solution of indole-3-acetic acid (auxin, IAA), 200 mM sodium chloride (salt, NaCl) and 25 µM solution of methyl viologen (MV)for 3h. The 500 ng of total RNA sample isolated from each tissue sample was processed for microarray analysis according to Affymetrix protocol. Two biological replicates for each sample (control, IAA, NaCl and MV) of wild-type and GRX overexpression line were used for microarray analysis.
Experiment type
transcription profiling by array 
Contacts
Mukesh Jain <mjain@nipgr.ac.in>, Jain Mukesh, Priya Pushp, Sharma Raghvendra
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-41963.idf.txt
Sample and data relationshipE-GEOD-41963.sdrf.txt
Raw data (1)E-GEOD-41963.raw.1.zip
Processed data (1)E-GEOD-41963.processed.1.zip
Array designA-AFFY-2.adf.txt
Links