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E-GEOD-41945 - Expression profiles of wildtype and Shox2 knockout embryonic limbs

Status
Released on 15 February 2013, last updated on 24 February 2013
Organism
Mus musculus
Samples (2)
Array (1)
Protocols (8)
Description
The development of vertebrate extremities is a complex process which requires a highly coordinated network of different transcriptional activities. The homeodomain transcription factor Shox2 is a key player in limb formation controlling neural, muscular and skeletal development. Here, we compared gene expression profiles of wildtype and Shox2 knockout limbs using microarray experiments to identify Shox2 target genes. Limbs of E12.5 mouse embryos were dissected, fore- and hindlimbs were pooled and genotyped for RNA extraction. RNA from 3 embryos of 2 different pregnancies (in total 6 embryos) was pooled per genotype (Wildtype and Shox2 Knockout) and compared.
Experiment type
transcription profiling by array 
Contacts
Carsten Sticht <geo@ncbi.nlm.nih.gov>, Anne Glaser, Norbert Gretz
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-41945.idf.txt
Sample and data relationshipE-GEOD-41945.sdrf.txt
Raw data (1)E-GEOD-41945.raw.1.zip
Processed data (1)E-GEOD-41945.processed.1.zip
Array designA-GEOD-8462.adf.txt
Links