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E-GEOD-41924 - Global analysis of off-target genes by GAPDH siRNA

Status
Released on 3 October 2013, last updated on 2 June 2014
Organism
Homo sapiens
Samples (3)
Array (1)
Protocols (5)
Description
A GAPDH siRNA was overexpressed in HeLa cells and microarray expression profiling was performed to experimentally identify the off-targets of this siRNA at the transcriptome level. Among all predicted siRNA off-targets by MirTarget2, the majority were downregulated by at least 10% (i.e. less than 90% remaining gene expression). Moreover, more than one fifth were downregulated by at least 30%. The gene knockdown data is consistent with previous observations that siRNA off-targets are generally silenced at a more moderate level as compared to silencing of the single intended target GAPDH siRNA was transfected into HeLa cells, and the unintended off-targets was identified by microarrays 24 hours post transfection.
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-41924.idf.txt
Sample and data relationshipE-GEOD-41924.sdrf.txt
Processed data (1)E-GEOD-41924.processed.1.zip
Additional data (1)E-GEOD-41924.additional.1.zip
Array designA-MEXP-1171.adf.txt
Links