E-GEOD-41923 - Distinct DNA methylomes of 2i and serum mouse ES cells are interconvertible and reflect pre- and post-implantation embryonic states
Released on 12 July 2013, last updated on 3 May 2014
This study describes the DNA methylation profiling using whole-genome bisulfite sequencing of mouse ES cells, either derived and maintained in 2i serum-free NDiff medium, or in the presence of serum and LIF, or maintained and derived in the presence of serum and LIF and subsequently adapted to 2i serum-free NDiff medium, or maintained and derived in the presence of 2i and LIF and subsequently adapted to 2i serum. DNA methylation profiling using whole-genome bisulfite sequencing of 14 samples, 3 different lines (E14, XT67E1, Rex/GFP-2i) of pluripotent mouse ES cells as well during conversion from 2i to serum and vice versa.
methylation profiling by high throughput sequencing
Arie B Brinkman, Ehsan Habibi, Filomena Matarese, Hendrik G Stunnenberg, Hendrik Marks, Hindrik H Kerstens, Isabelle Brun-Heath, Ivo G Gut, Joop H Jansen, Jorn Walter, Julia Arand, Konstantin Lepikhov, Leonie I Kroeze, Lucia Altucci, Marta Gut, Nina C Hubner, Rosaria Benedetti