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E-GEOD-41905 - A global transcriptome analysis of keratinocytes upon suppression of endogenous microRNA-31

Status
Released on 30 October 2012, last updated on 8 November 2012
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (8)
Description
MiR-31 is one of the most highly overexpressed miRNAs in psoriasis skin; however, its biological role in the disease has not been studied. Here we show that miR-31 is markedly overexpressed in psoriasis keratinocytes. To study the biological role of miR-31 in keratinocytes, we transfected miR-31 hairpin inhibitor (anti-miR-31) into primary human keratinocytes to inhibit endogenous miR-31. We performed a global transcriptome analysis of keratinocytes upon suppression of endogenous miR-31 using Affymetrix arrays. Expression profiling of primary human keratinocytes transfected with 10nM miR-31 hairpin inhibitor (anti-miR-31) or control hairpin RNA (anti-miR-Ctrl) for 48 hours (biological triplicates in each group) was performed using the Affymetrix GeneTitan system.
Experiment type
transcription profiling by array 
Contacts
Andor Pivarcsi, Cecilia Söderberg-Nauclér, Enikö Sonkoly, Florian Meisgen, Gangwen Han, Lynn M Butler, Mona Ståhle, Ning Xu, Xiao J Wang
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-41905.idf.txt
Sample and data relationshipE-GEOD-41905.sdrf.txt
Raw data (1)E-GEOD-41905.raw.1.zip
Processed data (1)E-GEOD-41905.processed.1.zip
Array designA-GEOD-11532.adf.txt
Links