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E-GEOD-41882 - Expression profiles in response to HMGA overexpression in late-stage neural precursor cells

Status
Released on 1 February 2013, last updated on 11 February 2013
Organism
Mus musculus
Samples (4)
Array (1)
Protocols (6)
Description
Neural precursor cells (NPCs) in the mammalian neocortex generate various neuronal and glial cell types in a developmental stage-dependent manner. Most neocortical NPCs lose their neurogenic potential after birth. We have previously shown that high mobility group A (HMGA) proteins confer the neurogenic potential on early-stage NPCs during the midgestation period, although the underlying mechanisms are not fully understood. Here we performed microarray analysis and compared expression profiles between control and HMGA2-overexpressed NPCs. Mouse neocortical neuroepithelial cells were isolated at embryonic day 11.5 and cultured as neurospheres for 9 days in vitro in the presence of fibroblast growth factor (FGF) 2 and epidermal growth factor (EGF). These cells were infected with control or HMGA2-expressing retrovirus, cultured in the presence of FGF and EGF for 3 days. Half of them were collected immediately for microarray analysis, and the other half were cultured in the absence of FGF for 12 h and then collected for microarray analysis.
Experiment type
transcription profiling by array 
Contacts
Yukiko Gotoh <ygotoh@iam.u-tokyo.ac.jp>, Yuki Fujii, Yusuke Kishi
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-41882.idf.txt
Sample and data relationshipE-GEOD-41882.sdrf.txt
Raw data (1)E-GEOD-41882.raw.1.zip
Processed data (1)E-GEOD-41882.processed.1.zip
Array designA-AFFY-130.adf.txt
Links