E-GEOD-41827 - Transcription profiling by array of HeLa cells after treatment with casiopeina II-gly
Released on 31 October 2012, last updated on 30 April 2015
Copper-based chemotherapeutic compounds Casiopeinas, have been presented as able to promote selective programmed cell death in cancer cells, thus being proper candidates for targeted cancer therapy. DNA fragmentation and apoptosis -in a process mediated by reactive oxygen species- for a number of tumor cells, have been argued to be the main mechanisms. However, a detailed functional mechanism (a model) is still to be defined and interrogated for a wide variety of cellular conditions; before establishing settings and parameters needed for their wide clinical application. Microarrays were used to determine the expression profile from HeLa cells in order to propose a model for the role played by intrinsic apoptosis triggered by the oxidative stress caused by Cas-II-gly. The cervix-uterine cell line HeLa was maintained at 37C in 5% CO2 under sterile conditions in Dulbecco's modified Eagle medium (DMEM, Sigma), supplemented with 10% fetal bovine serum (Sigma). Cells were treated with Cas II-gly in 96-well microplates by 6 h. HeLa cells whole genome gene expression experiments (triplicates for cases/controls) were performed in total mRNA extracted under the GPL570 protocol.
transcription profiling by array, compound treatment design
Karol Baca Lopez <firstname.lastname@example.org>, Adriana Vazquez-Aguirre, Alejandra I Valencia-Cruz, Anllely G Gutierrez, Carmen Mejia, Enrique Hernandez-Lemus, Karol Baca-Lopez, Laura I Uribe-Figueroa, Lena Ruiz-Azuara, Rodrigo Galindo-Murillo
Whole genome gene expression analysis reveals casiopeína-induced apoptosis pathways. Valencia-Cruz AI, Uribe-Figueroa LI, Galindo-Murillo R, Baca-López K, Gutiérrez AG, Vázquez-Aguirre A, Ruiz-Azuara L, Hernández-Lemus E, Mejía C.