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E-GEOD-41821 - A role for Prenylated rab acceptor 1 in vertebrate photoreceptor development

Status
Released on 2 November 2012, last updated on 8 November 2012
Organism
Mus musculus
Samples (32)
Array (1)
Protocols (7)
Description
The rd1 mouse retina is a well-studied model of retinal degeneration where rod photoreceptors undergo cell death beginning at postnatal day P10 until P21. This period coincides with photoreceptor terminal differentiation in a normal retina. We have used the rd1 retina as a model to investigate early molecular defects in developing rod photoreceptors prior to the onset of degeneration. Using a microarray approach, we performed gene profiling comparing rd1 and wild type retinas at four time points starting at P2, prior to any obvious biochemical or morphological differences, and concluding at P8, prior to the initiation of cell death. We have identified genes that are differentially regulated in the rd1 retina at early time points, which may give insights into developmental defects that precede photoreceptor cell death. This is the first report of PRA1 expression in the retina. Our data support the hypothesis that PRA1 plays an important role in vesicular trafficking between the Golgi and cilia in differentiating and mature rod photoreceptors. Retinal samples were harvested from both rd1/le and wt animals at postnatal days 2, 4, 6, and 8 for microarray. Each sample included 8-14 retinas and experiments were performed in quadruplicate. Ten micrograms of total RNA was used for cDNA systhesis in target molecule production.
Experiment type
transcription profiling by array 
Contacts
Matthew J Brooks <brooksma@mail.nih.gov>, Adnan Y Chowdhury, Alan J Mears, Ameair Abu Irqeba, Anand Swaroop, Angela M Richmond, Joshua G Martak, Judith M Ogilvie, Matthew J Brooks, Mohammed I Othman, Ritu Khanna, Sean C Hoge, Virginia M Dickison
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-41821.idf.txt
Sample and data relationshipE-GEOD-41821.sdrf.txt
Raw data (1)E-GEOD-41821.raw.1.zip
Processed data (1)E-GEOD-41821.processed.1.zip
Additional data (1)E-GEOD-41821.additional.1.zip
Array designA-AFFY-23.adf.txt
Links