E-GEOD-41420 - Gene expression profile in liver from rats fed with a diet containing genistein or daidzein or a control diet
Released on 10 October 2012, last updated on 1 October 2015
Effects of soy isoflavones, genistein and daidzein, on the hepatic gene expression profile and indices for lipid metabolism were compared in rats. The GeneChip data was normalized and summarized by using SuperNORM data service (Skylight Biotech Inc.). Significance of expressional change among groups was tested by 2-way ANOVA on the normalized CEL data, which was deposited in a tab-separated ASCII text format. Principal components were identified on the summarized gene data. Three groups of rats were fed with an experimental diet containing 2 g/kg of either genistein or daidzein, or a control diet free of isoflavone for 14 days. The basal composition of the experimental diet was (in g/kg): casein, 200; palm oil, 100; corn starch, 150; cellulose, 20; mineral mixture (AIN-93G), 35; vitamin mixture (AIN-93), 10; L-cystine, 3.0; choline bitartrate, 2.0 and sucrose to 1 kg.
transcription profiling by array
Tahashi Ide, Tomokazu Konishi, Tseye-Oidov Odbayar, Yoko Takahashi
A comparative analysis of genistein and daidzein in affecting lipid metabolism in rat liver. Takahashi Y, Odbayar TO, Ide T. , Europe PMC 19430610