E-GEOD-41355 - IRF-3, IRF-5, and IRF-7 coordinately regulate the type I IFN response in myeloid dendritic cells downstream of MAVS signaling

Status
Released on 23 April 2013, last updated on 2 June 2014
Organism
Mus musculus
Samples (29)
Array (1)
Protocols (7)
Description
Myeloid dendritic cells from WT, Irf3-/-xIrf7-/-, Irf3-/-xIrf5-/-xIrf7-/-, Mavs-/- (IPS1-/-)and Ifnar-/- mice were infected with West Nile virus to assess the contributions of specific signaling and transcription factors in initiating the antiviral response RPMI + rmGM-CSF purified bone marrow derived myeloid DC (mDC), mock and WNV infected with insect derived virus and RNA isolated at 24 hours post infection . Each genotype has matched mock and infected samples. n=6 for WT mock, WT infected, IFNAR mock, IFNAR infected, IRF3x7 infected; n=5 IRF3x7 infected; n=3 IRF3x5x7 mock, IRF3x5x7 infected, MAVS mock, MAVS infected. RNA was prepared using the Illumina bead station assay and hybridized to Illumina RefSeq-8 V2 BeadChips. Note: MAVS=IPS1
Experiment type
transcription profiling by array 
Contacts
Peter A Wilkinson <geo@ncbi.nlm.nih.gov>, Albert Huang, Alissa Lancaster, Ashlee V Moses, Courtney Wilkins, Helen M Lazear, Herbert W Virgin, Janko Nikolich-Zugich, Klaus Fruh, Larissa Thackray, Lisa Clepper, Margaret M Brassil, Mehul S Suthar, Michael GaleJr, Michael S Diamond, Sarah C Vick
Citation
IRF-3, IRF-5, and IRF-7 coordinately regulate the type I IFN response in myeloid dendritic cells downstream of MAVS signaling. Lazear HM, Lancaster A, Wilkins C, Suthar MS, Huang A, Vick SC, Clepper L, Thackray L, Brassil MM, Virgin HW, Nikolich-Zugich J, Moses AV, Gale M Jr, Fr�h K, Diamond MS. , Europe PMC 23300459
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-41355.idf.txt
Sample and data relationshipE-GEOD-41355.sdrf.txt
Raw data (1)E-GEOD-41355.raw.1.zip
Processed data (1)E-GEOD-41355.processed.1.zip
Additional data (1)E-GEOD-41355.additional.1.zip
Array designA-MEXP-1174.adf.txt
Links