E-GEOD-4093 - Resveratrol treatment of daf-16 mutant C. elegans
Submitted on 24 January 2006, released on 24 January 2006, last updated on 18 October 2011
Eggs were isolated by hypochlorite treatment and allowed to hatch in the absence of food as L1 stage arrested worms in S-buffer. Worms were then transferred to large plates containing NGM alone or NGM+ resveratrol and allowed to grow till young adult stage (ca 40 hours). Worms were harvested and total RNA extracted using trizol reagent. Poly-A+ RNA was isolated using Qiagen midi-prep kit and used for microarray hybridization. A compound treatment design type is where the response to administration of a compound or chemical (including biological compounds such as hormones) is assayed. Compound Based Treatment: Resveratrol Computed
unknown experiment type
Stanford Microarray Database <email@example.com>, Mohan Viswanathan
A role for SIR-2.1 regulation of ER stress response genes in determining C. elegans life span. Viswanathan M, Kim SK, Berdichevsky A, Guarente L.