E-GEOD-40885 - Data expression in alveolar macrophages induced by lipopolysaccharide in humans
Released on 15 September 2012, last updated on 30 September 2015
Rationale: Lipopolysaccharide (LPS) is ubiquitous in the environment. Inhalation of LPS has been implicated in the pathogenesis and/or severity of several lung diseases, including pneumonia, chronic obstructive pulmonary disease and asthma. Alveolar macrophages are the main resident leukocytes exposed to inhaled antigens. Objectives: To obtain insight into which innate immune pathways become activated within human alveolar macrophages upon exposure to LPS in vivo. In seven healthy humans sterile saline was instilled into a lung segment by bronchoscope, followed by instillation of LPS into the contralateral lung. Six hours later a bilateral bronchoalveolar lavage was performed and whole-genome transcriptional profiling was done (Affymetrix HG-U133 Plus 2.0) on purified alveolar macrophages, comparing cells exposed to saline or LPS from the same individuals.
transcription profiling by array
Frederic Reynier <email@example.com>, Alex F de Vos, Alexandre Pachot, Bruno Mougin, Jacobien J Hoogerwerf, Jaring S van der Zee, Malick Paye, Paul Bresser, Tom van der Poll
Gene expression profiles in alveolar macrophages induced by lipopolysaccharide in humans. Reynier F, De Vos AF, Hoogerwerf JJ, Bresser P, Van Der Zee JS, Paye M, Pachot A, Mougin B, Van Der Poll T. , Europe PMC 22952057