Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-4087 - Transcription profiling of mouse D4 and C2 ES cell lines assessed for chimerism and germline transmission

Status
Released on 12 June 2008, last updated on 12 October 2011
Organism
Mus musculus
Samples (34)
Arrays (2)
Protocols (8)
Description
Two independent ES cell lines (D4 and C2) were assessed for chimerism and germline transmission. Although these lines were able to produce chimerism at early passage, no germ line transmission was seen. After passaging in a proprietary medium and subcloning by detaching loosely connected cells and culturing in the recommended medium, both lines experienced an increase in potential as evidenced by an enhanced ability to go germ line. Analysis of early passage, and late passage detached and attached cells will allow the determination of genes that may be implicated in the increase of potential seen in these cells. Note: additional replicates may be added at a later date. Experiment Overall Design: this experiment include 6 samples and 34 replicates
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-4087.idf.txt
Sample and data relationshipE-GEOD-4087.sdrf.txt
Raw data (1)E-GEOD-4087.raw.1.zip
Processed data (1)E-GEOD-4087.processed.1.zip
Array designsA-AFFY-24.adf.txt, A-AFFY-36.adf.txt
R ExpressionSetE-GEOD-4087.eSet.r
Links