E-GEOD-40754 - Comparison of transcriptome of high differentiation-inducible murine erythroleukemia cells and low differentiation-inducible murine erythroleukemia cells

Status
Released on 23 April 2013, last updated on 2 June 2014
Organism
Mus musculus
Samples (96)
Array (1)
Protocols (7)
Description
Murine erythroleukemia (MEL) cells are differentiated by dimethyl sulfoxide (DMSO), hexamethylene bisacetamide (HMBA) or trichostatin A (TSA) treatment. We selsected high differentiation-inducible (HD) and low differentiation-inducible (LD)-MEL cells by recloning of original MEL cells. We screened erythroid differentiation related-genes to compare transcriptome of HD and LD-MEL cells. HD-MEL cells and LD-MEL cells were cultured 6, 12, 24, or 36 hours with 1.0% DMSO, 3.0 mM HMBA, or 15 nM TSA. The RNA of HD-MEL cells and LD-MEL cells were compare same time points of each drug treatment. The expression ratio (HD/LD) was calculated by average of technical quadruplicate microarray experiments includes two dye-swaps.
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-40754.idf.txt
Sample and data relationshipE-GEOD-40754.sdrf.txt
Raw data (1)E-GEOD-40754.raw.1.zip
Processed data (1)E-GEOD-40754.processed.1.zip
Array designA-GEOD-16035.adf.txt
Links