Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-4045 - Transcription profiling of human colorectal serrated adenocarcinomas and conventional adenocarcinomas reveals they are a molecular subclass as well as morphologically distinct
Submitted on 16 January 2006, released on 27 October 2007, last updated on 27 March 2012
Serrated adenocarcinomas are morphologically different from conventional adenocarcinomas. The serrated pathway has recently been proposed to represent a novel mechanism of colorectal cancer (CRC) formation. However, whether they are biologically different and truly form a distinct subclass of CRC, is not known. This study shows that the gene expression profile of serrated and conventional CRCs differs from each others and that serrated CRCs are not only morphologically novel, but also biologically distinct subclass of CRC. Experiment Overall Design: Total RNA was extracted from fresh frozen tumors with Trizol (GibcoBRL) and purified using RNeasy spin columns (Qiagen). The RNA quality was analyzed using a spectrophotometer and a Agilent 2100 Bioanalyzer (Agilent Technologies). Biotin labeled and fragmented cRNA was prepared from 8 micrograms of total RNA with procedures recommended for the HG-U133 GeneChip expression analysis (Affymetrix). The HG-U133A chips were hybridized, scanned and analyzed with Microarray Suite 5.0 software according to the manufacturer’s instructions (Affymetrix).
transcription profiling by array, co-expression, disease state
Serrated carcinomas form a subclass of colorectal cancer with distinct molecular basis. P Laiho, A Kokko, S Vanharanta, R Salovaara, H Sammalkorpi, H Järvinen, J-P Mecklin, T J Karttunen, K Tuppurainen, V Davalos, S Schwartz, D Arango, M J Mäkinen, L A Aaltonen. Oncogene 26(2):312-20 (2007)